Primary Human Aortic Smooth Muscle Cells (ACBRI 716) were initiated from normal human descending aortic tissue.
Cell Initiation
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. Each vial contains more than 1 x 106 cells.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
Companion Products
Each vial of cells is shipped with and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
These cells are qualified for use with:
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Standard Tests
| TEST | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
Miscellaneous Tests
| TEST | RESULTS |
| Cytoplasmic alpha-Actinin Filaments | > 95% positive by immunofluorescence |
| Cytoplasmic Desmin Intermediate Filaments | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | < 01% positive by immunofluorescence |
Characterization in Peer-Reviewed Literature
From Li et al Journal of Virology (2000) 74:6564-6569. Figure 5. Introduction and expression of the EGFP gene by the F-defective SeV vector in a variety of cell types in vitro. (A to C) GFP expression by primary neuronal cells derived from rat cerebral cortex 5 days after infection with the vector at an MOI of 5 at lower (A) and higher (C) magnification and immunostained with anti-MAP2 antibody (B). (D to I) Normal human hepatocytes (D and G), normal human lung microvascular endothelial cells (E and H) [ACBRI 468] , and normal human smooth muscle cells (F and I) [ACBRI 716] were infected with the F-defective SeV vector at an MOI of 3. GFP expression was observed 3 days after infection (G to I).
Select Citations
- Danesh et al. J Biological Chemistry, 2011.
- Li et al. Journal of Virology, 2000.
- Wickham et al. Nature Biotechnology, 1996
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
